When reporting experiments on animals, authors should indicate whether the institutional and national guide for the care and use of laboratory animals were followed. The work will be rejected if there is a lack of evidence.

Ethical approval

The study must be approved by the Ethical Committee/Institutional Review Board for Animal Experimentation (if possible) of the institute. Otherwise, it must be approved by the national or institutional Ethical Committee/Institutional Review Board.

Animal handling and experiments

The principle of laboratory animal care should be carried out according to the law of Animal Experiments Guidelines approved by the National Institute of Health (NIH)Guidelines of the European Council DirectiveNational Academy of Science or similar organization.  

The care must be taken to minimize the number of animals used for the experiments. All efforts are made to minimize any animal suffering. For the anesthetic purpose, chloroform or ether should be avoided. Instead, pentobarbital is preferred.

ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines are acceptable to improve the reporting of research using animals. The ARRIVE checklist is designed to be used while submitting manuscripts describing animal research. To complete the checklist, fill in the right-hand column with the section. If the checklist item is not applicable to the study being reported, provide a reason for not reporting this information in the right-hand column and will help reviewers and editors to ensure the high-quality reporting of animal research.

Oral acute toxicity study

The oral acute toxicity test must be conducted according to Organization for Economic Co-operation and Development (OECD) guidelines on laboratory animals. The maximum tested dose will be 5,000 mg/kg.