Detection of cytosolic tRNA in mammal by Northern blot analysis

  • Alshammari Fanar Hamad Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749, Korea.
  • Hye Young Jeong Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749, Korea.
  • Jong Hun Han Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749, Korea.
  • Irfan A. Rather Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749, Korea.
Keywords: Northern Blot, RNA, rRNA
DOI: 10.3329/bjp.v12i3.32501

References

Alwine JC, Kemp DJ, Stark GR. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA. 1977; 74: 5350-54.

Gesteland RF, Cech TR, Atkins JF. (eds.) The RNA World. 3rd ed. Cold Spring Harbor Laboratory Press. 2006.

Laneve P, Giangrande A. Enhanced Northern blot detection of small RNA species in Drosophila melanogaster. J Vis Exp. 2014; 9: e51814.

Wei Y. Northern blot of tRNA in yeast. Bio-protocol. 2013; 3: e464.

Published
2017-07-08

Apply citation style format of Bangladesh Journal of Pharmacology

Section
Visual Experiment
Financial Support
Self-funded
Conflict of Interest
Authors declare no conflict of interest
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