Antibacterial activity of Cathormion umbellatum

Keywords: Antibacterial activity, Antibiotic-resistant bacteria, Cathormion umbellatum
DOI: 10.3329/bjp.v16i3.53420

Abstract

The aim of this study was to determine the antibacterial activity of Cathormion umbellatum extracts against seven antibiotic-resistant bacteria. The pods, leaves and branches of C. umbellatum were extracted with ethanol and methanol. The disc diffusion assay was used to screen the antibacterial activity and broth microdilution and colorimetric assay were used to measure the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The result indicated that the highest inhibition zone (11 mm) was presented in ethanolic pods extract against multidrug resistance Klebsiella pneumoniae. The lowest MIC value of 0.05 mg/mL was obtained from branch extracted with ethanol against colistin resistant Pseudomonas aeruginosa. The lowest MBC values of 1.56 mg/mL were obtained when using C. umbellatum leaves extracted with methanol against all test antibiotic-resistant bacteria. This is the first report presented C. umbellatum extracts have the potential to eliminate antibiotic-resistant bacteria in patients. These findings show the antibacterial effect of C. umbellatum.

Introduction

Antibiotic-resistant bacteria are a consequence of improper and/or overuse of antibiotics (Naeim et al., 2020) which as the main causes of human death worldwide in the hospital (Abadi et al., 2019). Many antibiotic groups that bacteria were resisted such as β-lactams, aminoglycosides, ulfonamides, and fluoroquinolones (Huai et al., 2019). Some of the most life-threatening antibiotic-resistant bacterial strains with severe human implications worldwide are Pseudomonas aeruginosa, Acinetobacter baumannii (Abadi et al., 2019), Klebsiella pneumonia, Escherichia coli (Gregova and Kmet, 2020), Staphylococcus aureus (MRSA) (Bhattacharya, 2014), non-typhoidal Salmonella, Mycobacterium tuberculosis (Prestinaci et al., 2015), Stenotrophomonas maltophilia (Çıkman et al., 2016), Enterococcus faecalis (Miller et al., 2014), Proteus mirabilis (Tumbarello et al., 2012), and Burkholderia pseudomallei (Bugrysheva et al.,  2017). The finding for new drug sources to treat disease infected by antibiotic-resistant bacteria is required.

Medicinal plants are rich in a numerous variety of active compounds which have as antimicrobial properties such as berberine, piperine, eugenol, alicin, catechin, curcumin, saponins, tannins, alkaloids, alkenyl phenols, glycoalkaloids, flavonoids, sesquiterpenes lactones, terpenoids, and phorbol esters (Abdallah, 2011; Khameneh et al., 2019). The quinones from Lawsonia inermis had an antimicrobial activity against P. aeruginosa (Habbal et al., 2011). Hypericin from Hypericum perforatum, had general antimicrobial properties activity against methicillin-resistant and methicillin-sensitive Staphylococcus (Bahmani et al., 2019). PLR9 isolated from endophytic fungus Aspergillus neobridgeri shows antimicrobial activity against multi-drug resistant bacteria (Sadrati et al., 2020). Tannins isolated from the Pimenta dioica leaves show antimicrobial activity against methicillin resistant S. aureus (Al-Harbi et al., 2017). The aqueous extract of Lannea fruticosa showed the highest inhibition zone activity against both P. aeruginosa and P. mirabilis which was 20 mm and 19.5 mm, respectively (Kidane et al.,  2019).

Cathormion umbellatum (Vahl) Kosterm is a flowering plant in the legume family, Fabaceae which belongs to the mimosoid clade of the subfamily Caesalpinioideae. C. umbellatum is Thai mimosaceous plants that contained high antioxidant activity and can be stimulated white blood cell proliferation (Tunsaringkarn et al., 2014). Only antibacterial activity of C. umbellatum extracted with ethanol against E. coli was reported (Ramli, 2010). The determination of antibacterial activity against antibiotic-resistant bacteria has still lacked. Therefore, the aim of this study was to determine the antibacterial activity of C. umbellatum extracts against seven antibiotic-resistant bacteria collected from the Roi Et Hospital, Thailand.

Materials and Methods

Chemicals and reagents

Ethanol and methanol were purchased from QRëC™ (New Zealand). Dimethyl sulfoxide was purchased from Sigma–Aldrich (USA). Nutrient broth and bacterial agar were purchased from HiMedia (India). Iodonitrotetrazolium chloride was purchased from G-Biosciences (USA).

Plant materials and extraction

The fresh branch, leaves and pods of C. umbellatum were collected from Tha Muang Community, Tha Muang sub district, Selaphum District, Roi Et Province, Thailand. All plant samples were dried using hot air oven (POL-EKO-APARATURA company, Wodzisław Śląski, Poland) at 50ºC for 48 hours before were grounded into powder. The plant powder was extracted with ethanol and methanol with shaking for 3 hours and then filtered and evaporated using a rotary vacuum evaporator (BÜCHI Labortechnik AG, Switzerland). The percent yield was calculated (Rattanasuk and Phiwthong, 2021). The plant extracts were adjusted the final concentration to 500 mg/mL using dimethyl sulfoxide.

Antibacterial activity determination

The antibacterial activity of the C. umbellatum extracts was tested against seven antibiotic-resistant bacteria including A. baumannii, S. maltophilia, E. faecalis, B. pseudomallei, P. mirabilis, multidrug resistance K. pneumoniae, colistin resistant P. aeruginosa. The active bacterial cultures were adjusted the cell concentration at OD600 to 0.1 before used.

The antibacterial activity of C. umbellatum extract was primary determined using disc diffusion assay (Boongapim et al., 2021; Malaka et al., 2018). Ten microliters of each C. umbellatum extract (500 mg/mL) was dropped onto the center of the paper disc. The dimethyl sulfoxide was used as a negative control. The bacterial culture plates were incubated at 37ºC for 24 hours. The inhibition zone formation around the paper disc indicated as antibacterial activity of C. umbellatum extracts were measured.

The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of C. umbellatum extracts were determined using a broth microdilution and colorimetric assay (Rattanasuk and Phiwthong, 2020). The C. umbellatum extracts which presented the inhibition zone from the previous part were 2-fold serial diluted in a 96-well plate containing NB.  The 96-well bacterial culture plates were incubated at 37ºC for 24 hours. The iodonitrotetrazolium chloride (4 mg/mL) solution was added into each well of the 96-well bacterial culture plate and then incubated at 37ºC for 1 hour. The MIC was referred to as the lowest concentration of the C. umbellatum extract that can inhibit bacterial growth. The MBC was considered as the lowest concentration of C. umbellatum extract that can eliminate the bacteria that did not produce a color change after the addition of iodonitrotetrazolium chloride  (Dzotam et al., 2016).

Results

Percent yield and inhibition zone

The result of percent yield indicated that the highest percent yields at 15.9% was obtained when used the C. umbellatum leaves extracted with methanol, followed by pods extracted with methanol (13.3%) and leaves extracted with ethanol (11.7%), respectively. The lowest percent yields at 4.9% was found in branch extracted with ethanol.

The result of disc diffusion assay indicated that the highest inhibition zone at 11 mm was presented in ethanolic pods extract against multidrug resistance K. pneumoniae, followed by pods extracted with ethanol (10 mm), branch extracted with ethanol (9.5 mm) and leave extracted with methanol (9 mm) against B. pseudomallei, P. mirabilis and colistin resistant P. aeruginosa, respectively (Table I).

Table I: Inhibition zone (mm)

  Pod Leaf Branch
  Ethanol Methanol Ethanol Methanol Ethanol Methanol
A. baumannii 7 7 6.5 7 7.5 6.6
S. maltophilia 7.5 7.5 6.5 7 7 7.5
E. faecalis 7 8 8 7 8.5 7
B. pseudomallei 10 7.5 6.5 7.5 7.5 6.5
P. mirabilis 7 8 8 8 9.5 6.5
Multidrug resistant K. pneumoniae 11 7.5 7.5 8 8 7
Colistin resistant P. aeruginosa 7.5 7.5 8 9 7.5 8

MIC and MBC values

The results indicated that the lowest MIC value of 0.1 mg/mL against colistin resistant P. aeruginosa was obtained from branch extracted with ethanol followed by 0.1 mg/mL was obtained from leave extracted with methanol against P. mirabilis, pods and leave extracted with methanol against B. pseudomallei (0.4 mg/mL), respectively (Table II). The lowest MBC values of 1.6 mg/mL were obtained when using C. umbellatum leaves extracted with methanol against all test antibiotic-resistant bacteria, leaves extracted with ethanol against S. maltophilia, B. pseudomallei P. mirabilis and colistin resistant P. aeruginosa, pods extracted with ethanol against A. baumannii, S. maltophilia, E. faecalis and B. pseudomallei, pods extracted with methanol against S. maltophilia, E. faecalis, B. pseudomallei and P. mirabilis. The highest MBC value of 12.5 mg/mL was found in branch extracted with methanol against E. faecalis.

Table II: Minimum inhibitory concentrations and minimal bactericidal concentration

  Minimum inhibitory concentrations (mg/mL)
  Pod Leaf Branch
  Ethanol Methanol Ethanol Methanol Ethanol Methanol
A. baumannii 0.8 0.8 0.8 0.8 1.6 1.6
S. maltophilia 0.8 0.8 0.8 0.8 3.1 3.1
E. faecalis 0.8 0.8 1.6 0.2 3.1 3.1
B. pseudomallei 0.4 0.2 0.4 0.8 1.6 1.6
P. mirabilis 0.8 0.8 0.8 0.1 1.6 3.1
Multidrug resistant K. pneumoniae 0.8 1.6 1.6 0.8 3.1 3.1
Colistin resistant P. aeruginosa 1.6 1.6 0.8 0.8 0.1 1.6
  Minimal bactericidal concentration (mg/mL)
A. baumannii 1.6 3.1 3.1 1.6 3.1 3.1
S. maltophilia 1.6 1.6 1.6 1.6 6.3 6.3
E. faecalis 1.6 1.6 3.1 1.6 6.3 12.5
B. pseudomallei 1.6 1.6 1.6 1.6 3.1 3.1
P. mirabilis 3.1 1.6 1.6 1.6 3.1 6.3
Multidrug resistant K. pneumoniae 3.1 6.3 3.1 1.6 6.3 6.3
Colistin resistant P. aeruginosa 3.1 3.1 1.6 1.6 3.1 3.1

Discussion

C. umbellatum shows antibacterial and antioxidant activity (Tunsaringkarn et al., 2014). It has been found that the ethanolic branch extract was presented that the lowest MIC value of 0.1 mg/mL against CoR-PA and methanolic leave extract was showed the lowest MBC values of 1.6 mg/mL against all test antibiotic-resistant bacteria. The mechanism of action is not clear.

The are no reports about that antibacterial activity C. umbellatum extract against antibiotic-resistant bacteria. Only a report about the ethanolic extract of C. umbellatum leaves has a MIC value of 0.8 mg/mL against S. aureus, B. subtilis and E. coli is presented (Ramli, 2010). The C. umbellatum extract is presented high antibiotic potential activity due to lower MIC values compared with using antibiotics (Kawamura-Sato et al., 2000) or Lannea fruticose (Kidane et al., 2019), Tanacetum vulgare and Bidens sulphurea extract (Chiavari-Frederico et al., 2020). The present study indicates that the MIC values of C. umbellatum pods and leave extracted with methanol against B. pseudomallei are lower than MIC values of amoxicillin-clavulanic acid (8 mg/mL), ceftazidime (8 mg/mL), imipenem (2 mg/mL), meropenem (2 mg/mL), doxycycline (2 mg/mL), tetracycline (8 mg/mL), chloramphenicol (8 mg/mL) and trimethoprim-sulfamethoxazole (4 mg/mL) (Karatuna et al., 2020).

The MBC values of C. umbellatum extract against A. baumannii from this research are higher than MBC of colistin (0.5 mg/L) and sulbactam (32 mg/L) (Thamlikitkul and Tiengrim, 2014). The Litsea cubeba oil exhibits a strong inhibitory effect with MBC value of 0.125% (v/v) against S. maltophilia (Zhang et al., 2020). Red honey and white honey have MBC values of 30.4-62.5% and 60.7-75 % (v/v) against multidrug resistant bacteria (Wasihun and Kasa, 2016). Proanthocyanidins and flavonoids glycoside are potential phytochemical groups content of C. umbellatum extracts which act as an antibacterial reagent (Ramli, 2010). Proanthocyanidins are phytochemicals found from C. umbellatum which synthesized from tannin and are presented various biological activities including antioxidant, anticancer, antidiabetic, neuroprotective, and antimicrobial activity (Rauf et al., 2019). The proanthocyanidins of Dalbergia monetaria extracts present the antibacterial activity against methicillin sensitive S. aureus, methicillin-resistant S. aureus and P. aeruginosa with MIC values of 64, 64 and 32 μg/mL, respectively (de Moura et al., 2020). The curcuminoids have an antimicrobial activity against E. faecalis with MBC of 50 μg/mL (Suttipalin et al., 2014).

Conclusion

The present study shows the antimicrobial activity of C. umbellatum against antibiotic-resistant bacteria.

Author Biography

Surachai Rattanasuk, Department of Science and Technology, Faculty of Liberal Arts and Science, Roi Et Rajabhat University, Selaphum, Roi Et 45120, Thailand.

Assistant Professor

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Published
2021-07-12

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Research Articles
Financial Support
This research project was supported by Roi Et Rajabhat University grant No. 2557A15062001
Conflict of Interest
Authors declare no conflict of interest
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