Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line

Keywords: Apoptosis, β-glucan, Cell viability, DNA damage, ELISA, SH-SY5Y cell, XTT assay
DOI: 10.3329/bjp.v16i4.54872


Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxydesoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. β-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that β-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, β-glucan treatment did not change the BCL-2 protein level. Altogether, β-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis.


Neuroblastoma, the most prevalent extra-brain solid tumor of infancy, is the primary reason of death in children. More than 40 percent of patients are noted to be at excessive risk, including children suffering metastatic disease (Moreno et al., 2017). Despite the existence of multiple therapeutic approaches, including immunotherapy, surgery, chemotherapy, and radiotherapy, the resistance and recurrence of neuroblastoma often occur, necessitating an alternative effective therapy of the cancer (Suebsoonthron et al., 2017). About a third of patients are resistant to first line treatment and have very bad consequences (Ladenstein et al., 2008). In addition, because of the intensity of the multimodal therapy, survivors are exposed to considerable stress from long-term effects (Laverdière et al., 2005). Late treatment toxicities, such as the emergence of secondary cancers, put neuroblastoma patients at risk of serious or life-threatening diseases, emphasizing the need for extensive and effective preclinical drug screening (Nolan et al., 2020). More research into the discovery of new therapeutic agents that reduce mortality due to neuroblastoma while also having less side effects is needed in this context. SH-SY5Y cells are human neuroblastoma cells obtained from a 4-year-old female's bone marrow biopsy with neuroblastoma. SH-SY5Y cells are frequently used as a model for neuronal activity and differentiation in vitro (Suebsoonthron et al., 2017). Food items and natural ingredients have long been used as health treatments thanks to the widespread belief that they have mild side effects. As a result, insights into the mechanisms by which foods and natural ingredients can have potential beneficial effects is necessary for human societies.

β-Glucans are a type of polysaccharide that is distinguished by its position in the cell wall. It is abundant in barley and oats (Mantovani et al., 2008). Many illnesses, including atherosclerosis, infectious disease, hyperlipidemia, diabetes, cancer, and obesity, are now known to benefit from β-glucans in the prevention and treatment. Different properties of β-glucans, such as immune activation and bile acid trapping, mediate the effects (Chen, 2013; Chen and Huang, 2009). β-Glucan administration has been confirmed to cause substantial stimulation of reticuloendothelial system phagocytic activity, with subsequent enhancement of host defense mechanisms, since the 1970s (Sima et al., 2019). Granulocytes, monocytes, dendritic cells, and macrophages were reported to have higher proliferative activity and phagocytic function, along with stimulation of cytokine production (including such interleukin-1, -2, and -6) (Quinn, 1990). Increased generation of these cytokines activates immune effector cells, leading to an improvement in anti-infectious and, in particular, anti-cancer immunity (Vannucci et al., 2013). β-Glucan therapy has been shown to be effective against a variety of cancers in both human and animal models (Sima et al., 2019). Two key β-glucan receptors, lactosylceramide and receptor 3 (CR3), mediate its anti-cancer properties. Immune boosting, cancer-prevention and direct tumor suppession behaviors are all related to anti-cancer effects of β-glucan (Choromanska et al., 2015). Both soluble and particulate β-glucans isolated from S. cerevisae inhibited the growth of B16 melanoma and breast carcinoma cells, and also increased the longevity of mice implanted with subcutaneous tumors (di Luzio et al., 1979).

Lentinula edodes-derived β-glucan was shown to have good antitumor activity against sarcoma 180 in vivo studies (Zhang et al., 2005). In the treatment of human neuroblastoma xenografts, it was shown that,3-1,4-beta-glucan synergized with anti-GD2 monoclonal antibody (MoAb) 3F8 (mouse IgG3) (Cheung et al., 2002). Relapse-free survival was 80-10% at 24 months in a phase 1 study of a bivalent gangliosides vaccine in combination with β -glucan. The vaccine and β -glucan were well tolerated (Kushner et al., 2014). Several works have demonstrated the anti-cancer effects of β-glucan on a variety of cancer cell lines yet the mechanisms involved in anti-cancer effect of β-glucan against neuroblastoma remains unknown. The goal of this research was to investigate the cytotoxic activity of β-glucans on the SH-SY5Y cell line, as well as the mechanisms involved in this activity.

Materials and Methods

Cell culture and cell lines

SH-SY5Y cell line (neuroblastoma) and L929 cell line (mouse fibroblast) were purchased from the American Type Culture Collection (ATCC, USA). SH-SY5Y and L929 cell lines were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich) and placed in  humidified atmosphere of 5% CO2 incubator at 37 ºC. 10% fetal bovine serum (FBS) (Sigma-Aldrich) and 1% antibiotic mixtures of penicillin and streptomycin were added to this medium. Before the procedure, the L. edodes–derived β–glucan (lentinan)  (Sigma-Aldrich) was dissolved in DMSO (dimethyl sulfoxide)  and diluted in the culture medium to a final DMSO concentration of less than 0.1 percent.

Examination of 8-oxo-dG, cleaved caspase 3, BCL-2, cleaved PARP and Bax levels

The human ELISA kits of 8-hydroxydesoxyguanosine (8-oxo-dG) (BT Lab, catalog #E1436HU), cleaved caspase 3 (BT Lab, catalog # E6970HU), BCL-2 (BT Lab, catalog #E1832HU), cleaved PARP (BT Lab, catalog #E6971HU) and Bax (BT Lab, catalog #E1825HU) were used to assess the levels of 8-oxo-dG, cleaved caspase 3, BCL-2, cleaved PARP and Bax in β-glucan-treated and untreated SH-SY5Y cells. In short, SH-SY5Y cells were cultivated into a 6-well plate and treated with 125 μg/mL β-glucan for 24 hours. SH-SY5Y cells that had been treated with β-glucan and those that had not were gathered and diluted in PBS. Then they were frozen and thawed three times. Following that, the quantities of  8-oxo-dG, cleaved caspase 3, BCL-2, cleaved PARP and Bax in cell lysates were measured following the manufacturer’s directions. Bradford  protein  assay  kit  (Merck Millipore, Germany) was used to assess the total protein quantities in β-glucan-treated and untreated SH-SY5Y cells (Ergul and Bakar-Ates, 2021).

Total antioxidant status and total oxidant status measurement in SH-SY5Y cells

The total antioxidant status assay kit (Rel Assay Diag-nostics, Turkey) and the total oxidant status assay Kit (Rel Assay Diagnostics, Turkey) were used to examine total antioxidant status and total oxidant status levels in β-glucan-treated and untreated SH-SY5Y cells, respectively. SH-SY5Y cells were treated with 125 μg/mL β-glucan for 24 hours and the manufacturer’s directions were implemented. For total antioxidant status and total oxidant status, the data were expressed as mmol Trolox Equiv./L and mol H2O2 Equiv./L, respectively (Taskiran and Ergul, 2021).

Statistical analysis

The laboratory findings were stated as mean ± standard error. The results obtained from the cell viability tests were examined using the one way ANOVA test with post hoc test. The results obtained from 8-oxo-dG, cleaved caspase 3, BCL-2, cleaved PARP and Bax, total antioxidant status and total oxidant status levels tests were examined using Independent Samples t Test. A p-value of less than 0.01 was used to accept statistically significant differences. GraphPad Prism 8.0 software (USA) was used for data analysis and graphical presentations.


Cytotoxic effect of β-glucan on SH-SY5Y cells

In the beginning, the cytotoxic activity of β-glucan was investigated in SH-SY5Y cells. At 62.5 μg/mL and higher doses, β-glucan significantly inhibited the growth of SH-SY5Y cells as compared to control (P < 0.01). The IC50 value of β-glucan in SH-SY5Y cells was determined to be 94.6 μg/mL for 24 hours (Figure 1a). In addition, the cytotoxic activity of β-glucan was tested in L929, a non-cancerous cells, and the results demonstrated that β-glucan did not produce cytotoxic effect on L929 cells  (Figure 1b).

Figure 1: The antiproliferative effects of β-glucan on SH-SY5Y cells (a) and L929 cells (b). The findings are calculated as a percentage of viable cells versus control. The results are presented as the mean ± SEM of three samples. ap<0.01 as compared to the control group

Effect of β-glucan on Bax, cleaved caspase 3, BCL-2 and cleaved PARP levels in SH-SY5Y cells

The ELISA technique was used to determine the protein expressions in SH-SY5Y cells, such as Bax, cleaved caspase 3, cleaved PARP, and BCL-2, all of which are involved in apoptosis (Figure 2). Treatment with 125 μg/mL of β-glucan for 24 hours significantly increased the levels of Bax (p<0.01), cleaved PARP (p<0.05) and cleaved caspase 3 (p<0.05). β-glucan, on the other hand, did not alter the BCL-2 level (p>0.05).

Figure 2: β-glucan (125 μg/mL) enhanced apoptosis of SH-SY5Y cells. The cleaved caspase 3, BCL-2, cleaved PARP and Bax levels were calculated using the ELISA kits. Results are represented as mean ± SEM of three samples. ap< 0.01 and bp<0.05 as compared to the control group

Effect of β-glucan on 8-oxo-dG level in SH-SY5Y cells

The ELISA technique was also used to determine 8-oxo-dG expressions in SH-SY5Y cells in order to assess the DNA-damaging effects of β-glucan. Treatment with 125 μg/mL of β-glucan for 24 hours significantly increased the level of 8-oxo-dG (p<0.01) (Figure 3).

Effect of β-glucan on total antioxidant status and total oxidant status levels in SH-SY5Y cells

Figure 3: β-glucan (125 μg/mL) enhanced DNA damage of SH-SY5Y cells. The 8-oxo-dG level was calculated using the ELISA kit. Results are represented as mean ± SEM of three samples. ap<0.01 as compared to the control group

The effect of β-glucan on total antioxidant status and total oxidant status  in SH-SY5Y cells was assessed using total antioxidant status and total oxidant status assay kits. β-glucan administration significantly increased total oxidant status level (p<0.05).

On the other hand, total antioxidant status  level did not change in β-glucan-treated cells (p>0.05). Total antioxidant status was determined as 0.48 ± 0.02 in the control group and 0.47 ± 0.00 in 125 μg/mL β-glucan-treated cells. Additionally, total oxidant status was found to be as 26.96 ± 5.46 in the control group and 57.20 ± 3.86 in 125 μg /mL β-glucan-treated cells (Figure 4).

Figure 4: β-glucan at dose of 125 μg/mL didn’t not cause a significant change in the total antioxidant status level (A), but increased the total oxidant status level (B) of SH-SY5Y cells significantly. Results are represented as mean ± SEM of three samples. ap<0.05 as compared to the control group


β-Glucan had a concentration-dependent cytotoxic effect on SH-SY5Y cells. It significantly suppressed SH-SY5Y cell reproduction in a concentration-dependent way, with an IC50 value of 94.6 μg/mL after 24 hours. β-Glucan cytotoxic effects on non-cancerous L929 cells were studied in this context, and the findings showed that β-glucan had no significant cytotoxic effects on L929 cells.

Apoptosis is well understood to play a key role in the cellular pathogenesis of tumors and to impact the result of pharmacological treatments (Chen et al., 2018). The levels of cleaved PARP, cleaved caspase 3, Bax and  BCL-2 were measured using ELISA to confirm the apoptotic effect of β-glucan on SH-SY5Y cells. One of the defense mechanisms against the growth and progression of tumors is the initiation of cell apoptosis, which is a major focus of cancer treatment (Tang et al., 2018). Pro-apoptotic and anti-apoptotic proteins are involved the BCL-2 protein family, and the ratio of pro-apoptotic versus anti-apoptotic proteins is widely used to determine cell fate (Ergul and Bakar-Ates, 2020). Apoptosis-inducing protein Bax interrupts the mitochondrial membrane, releasing cytochrome c, which generates the apoptosome complex. This complex triggers apoptosis by activating effector caspases. BCL-2, on the other hand, maintains membrane stability, prevents apoptosis, and inhibits cytochrome c release (Baig et al., 2016).

Caspases are a group of enzymes that are essential apoptosis effectors, and their stimulation is a hallmark of the process. They are generated as zymogens, that can be split to produce active enzymes when apoptosis is induced (Jin and El-Deiry, 2005). Caspases 3 is an executioner caspase that cleaves a variety of cellular substrates, resulting in a variety of morphological and biochemical alterations in apoptotic cells (Elmore, 2007).

Furthermore, PARP is a critical component of DNA repair pathways, especially in the repair of base excisions, and its cleavage or suppression causes cell death via exploiting a DNA repair defect (Sachdev et al., 2019). This compound is among the most well-studied activated caspase substrates (Tang et al., 2018). In this research, 125 μg/mL β-glucan treatment significantly increased pro-apoptotic Bax, cleaved caspase 3, and cleaved PARP protein expressions, while not alerting anti-apoptotic BCL-2 expression. The 8-oxo-dG ELISA method was used to look at DNA fragmentation in SH-SY5Y cells after 24 hours of β-glucan treatment to see if the cytotoxic effect of β-glucan is linked to DNA damage.

8-oxo-dG is a well-known biomarker for DNA oxidative damage (Guo et al., 2016). β-Glucan treatment at 125 μg/mL significantly increased 8- oxo-dG quantities in SH-SY5Y cells, implying that β-glucan has cytotoxic and apoptotic properties.

Most anti-cancer drugs work by causing oxidative stress in tumor cells, which is thought to be the cause of most macromolecular alterations throughout the cell. Proteins, membrane lipids, and DNA are among the macromolecules that reactive oxygen species can target (Matos et al., 2019). The total oxidant status is one of several parameters used to estimate oxidative stress and therefore is commonly used to assess the overall oxidative status of cells. The total antioxidant status is also used to assess the overall antioxidant status of cells (Ergul and Bakar-Ates, 2020). In this context, we examined whether β-glucan could cause cytotoxicity by raising total oxidant status levels. In comparison to untreated cells, exposure to β-glucan for 24 hours increased total oxidant status levels; but, there was no significant variation in total antioxidant status levels. Treatment with β-glucan caused a rise in total oxidant status but no alteration in total antioxidant status levels, indicating that oxidative stress was induced in the β-glucan-treated SH-SY5Y cells. Furthermore, the damage of mitochondrial membrane and excessive increase of reactive oxygen species can activate the intrinsic apoptosis pathway (Lee et al., 2019).

In light of our mitochondrial membrane potential results, it can be conclude that glucosamine sulfate promotes apoptosis via the mitochondrial route. In agreement with our results, It was shown that β-glucan isolated from L. edodes had a remarkable inhibition rate of 75% on S-180 cell viability, which was even higher than that of the positive control of cytoxan (54%). β-Glucan facilitated the accumulation of immune cells into tumors, which was followed by cell apoptosis and inhibition of cell viability. β-Glucan also down-regulated PARP1 and the anti-apoptotic protein Bcl-2 expressions in cancer cells, while up-regulating the tumor suppressor p53 and pro-apoptotic proteins Bax and caspase 3/9 (Xu et al., 2016). β-Glucan from oats had antitumor activity and triggered oxidative stress in human lung cancer cell lines. Normal cells, on the other hand, showed no signs of toxicity (Choromanska et al., 2018). It was demonstrated  that pulmonary exposure to CSBG (a soluble cell wall β-glucan derived from C. albicans) significantly increased apoptosis and raised the accumulation of 8-OHdG in the lung parenchyma (Inoue et al., 2009). Zhang et al. (2006) also used a water-soluble β-glucan derived from Poria cocos mycelia (PCM3-II). The growth-inhibitory activity of PCM3-II was investigated further in vitro using human breast cancer MCF-7 cells. MTT analysis revealed that PCM3-II decreased MCF-7 cell viability in a dose-dependent manner. At 400 μg/mL PCM3-II, cancer cell viability was reduced by 50% compared to the control. The PCM3-II also caused a reduction of the anti-apoptotic Bcl-2 protein, but not the proapoptotic Bax protein, resulting in an increase in the Bax/Bcl-2 ratio in breast cancer cells (Zhang et al., 2006). Breast cancer cells have also been shown to be inhibited by LNT. Further exploration discovered that LNT administration induced the cleavage of Poly (ADP-ribose) polymerase (PARP) (Zhang et al., 2020). β-Glucan showed cytotoxic effects on colon cancer cells by using MTT assay. At 200 μg/mL dose, the viability of cancer cells was reduced by about 50% (Kim et al., 2009).  Lentinan (1-6,1-3)- β-glucan) suppressed the viability of C6 glioma cells by inducing apoptosis, blocking the cell cycle, increasing the rate of cells in the G0/G1 stage, and decreasing the rate of cells in the S-stage (Atiq and Parhar, 2020). It was also demonstrated that lentinan showed synergistic effects with oxaliplatin in inhibiting NF-kB, STAT3, and survivin expression in Hep-G2 cells (human hepatocyte carcinoma) in mice with H22 tumors (Zhang et al., 2016). Lentinan treatment stimulates autophagy and programmed cell death in human osteosarcoma cells by inhibiting MAPK/ERK signaling and up-regulating miR-340 in MG63 cells. LNT raises Beclin-1, LC3B-II/LC3B-I, and caspase-3, -9 amounts while decreasing cyclin D1 amounts (Xu et al., 2018). It was recently discovered that schizophyllan (-1,3 β-glucan) therapy inhibits the growth of rat CNS-1 glioma cells by inhibiting cell cycle and programmed cell death by p53-mediated inhibition (Atiq and Parhar, 2020). As a result, natural compounds would be effective anti-glioblastoma drugs, either alone or in combination with other anti-cancer drugs to improve their sensitivity to glioblastoma. However, due to the exis-tence of blood-brain barrier, the most significant obstacle in the treatment of GBM is insufficient drug delivery. The role of modified formulations such as liposomes, nanoparticles and drug conjugates in overcoming the BBB and improving drug delivery for the management of glioblastoma multiforme was high-lighted (Atiq and Parhar, 2020).


Fungi derived β-glucan significantly suppressed SH-SY5Y cell reproduction in a concentration-dependent way without causing significant cytotoxic effects on L929 cells. β-glucan treatment significantly increased pro-apoptotic Bax, cleaved caspase 3, and cleaved PARP protein expressions. β-Glucan treatment also significantly increased 8- oxo-dG quantities in SH-SY5Y cells and thus the cytotoxic effect of β-glucan may be linked to DNA damage. Treatment with β-glucan caused a rise in total oxidant status supporting its cytotoxic effects.


Atiq A, Parhar I. Anti-neoplastic potential of flavonoids and polysaccharide phytochemicals in Glioblastoma. Molecules 2020; 25: 4895.

Baig S, Seevasant I, Mohamad J, Mukheem A, Huri HZ, Kamarul T. Potential of apoptotic pathway-targeted cancer therapeutic research: Where do we stand? Cell Death Dis. 2016; 7: e2058.

Chen J. Recent advance in the studies of beta-glucans for cancer therapy. Anticancer Agents Med Chem. 2013; 13: 679-80.

Chen J, Huang XF. The effects of diets enriched in beta-glucans on blood lipoprotein concentrations. J Clin Lipidol. 2009; 3: 154-58.

Chen Z, Zhang B, Gao F, Shi R. Modulation of G2/M cell cycle arrest and apoptosis by luteolin in human colon cancer cells and xenografts. Oncol Lett. 2018; 15: 1559-65.

Cheung NK, Modak S. Oral (1--3),(1--4)-beta-D-glucan synergizes with antiganglioside GD2 monoclonal antibody 3F8 in the therapy of neuroblastoma. Clin Cancer Res. 2002; 8: 1217-23.

Choromanska A, Kulbacka J, Harasym J, Oledzki R, Szewczyk A, Saczko J. High- and low-molecular weight oat beta-glucan reveals antitumor activity in human epithelial lung cancer. Pathol Oncol Res. 2018; 24: 583-92.

Choromanska A, Kulbacka J, Rembialkowska N, Pilat J, Oledzki R, Harasym J, Saczko J. Anticancer properties of low molecular weight oat beta-glucan: An in vitro study. Int J Biol Macromol. 2015; 80: 23-28.

Di Luzio NR, Williams DL, McNamee RB, Edwards BF, Kitahama A. Comparative tumor-inhibitory and antibacte-rial activity of soluble and particulate glucan. Int J Cancer. 1979; 24: 773-79.

Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol. 2007; 35: 495-516.

Ergul M, Bakar-Ates F. A specific inhibitor of polo-like kinase 1, GSK461364A, suppresses proliferation of Raji Burkitt's lymphoma cells through mediating cell cycle arrest, DNA damage, and apoptosis. Chem Biol Interact. 2020; 332: 109288.

Ergul M, Bakar-Ates F. Investigation of molecular mechanisms underlying the antiproliferative effects of colchicine against PC3 prostate cancer cells. Toxicol In Vitro. 2021; 73: 105138.

Guo C, Li X, Wang R, Yu J, Ye M, Mao L, Zhang S, Zheng S. Association between oxidative DNA damage and risk of colorectal cancer: Sensitive determination of urinary 8-hydroxy-2'-deoxyguanosine by UPLC-MS/MS analysis. Sci Rep. 2016; 6: 32581.

Inoue K, Takano H, Oda T, Yanagisawa R, Tamura H, Adachi Y, Ishibashi K, Ohno N. Soluble cell wall beta-glucan of Candida induces/enhances apoptosis and oxidative stress in murine lung. Immunopharmacol Immunotoxicol. 2009; 31: 140-45.

Jin Z, El-Deiry WS. Overview of cell death signaling pathways. Cancer Biol Ther. 2005; 4: 139-63.

Kim MJ, Hong SY, Kim SK, Cheong C, Park HJ, Chun HK, Jang KH, Yoon BD, Kim CH, Kang SA. beta-Glucan enhanced apoptosis in human colon cancer cells SNU-C4. Nutr Res Pract. 2009; 3: 180-84.

Kushner BH, Cheung IY, Modak S, Kramer K, Ragupathi G, Cheung NK. Phase I trial of a bivalent gangliosides vaccine in combination with β-glucan for high-risk neuroblastoma in second or later remission. Clin Cancer Res. 2014; 20: 1375-82.

Ladenstein R, Pötschger U, Hartman O, Pearson AD, Klingebiel T, Castel V, Yaniv I, Demirer T, Dini G, EBMT Paediatric Working Party. 28 Years of high-dose therapy and SCT for neuroblastoma in Europe: Lessons from more than 4000 procedures. Bone Marrow Transplant. 2008; 41: S118-27.

Laverdière C, Cheung NK, Kushner BH, Kramer K, Modak S, LaQuaglia MP, Wolden S, Ness KK, Gurney JG, Sklar CA. Long-term complications in survivors of advanced stage neuroblastoma. Pediatr Blood Cancer. 2005; 45: 324-32.

Lee SI, Jeong YJ, Yu AR, Kwak HJ, Cha JY, Kang I, Yeo EJ. Carfilzomib enhances cisplatin-induced apoptosis in SK-N-BE(2)-M17 human neuroblastoma cells. Sci Rep. 2019; 9: 5039.

Mantovani MS, Bellini MF, Angeli JP, Oliveira RJ, Silva AF, Ribeiro LR. beta-Glucans in promoting health: Prevention against mutation and cancer. Mutat Res. 2008; 658: 154-61.

Matos CP, Adiguzel Z, Yildizhan Y, Cevatemre B, Onder TB, Cevik O, Nunes P, Ferreira LP, Carvalho MD, Campos DL, Pavan FR, Pessoa JC, Garcia MH, Tomaz AI, Correia I, Acilan C. May iron (III) complexes containing phenanthroline derivatives as ligands be prospective anticancer agents? Eur J Med Chem. 2019; 176: 492-512.

Moreno L, Caron H, Geoerger B, Eggert A, Schleiermacher G, Brock P, Valteau-Couanet D, Chesler L, Schulte JH, De Preter K, Molenaar J, Schramm A, Eilers M, Van Maerken T, Johnsen JI, Garrett M, George SL, Tweddle DA, Kogner P, Berthold F, Koster J, Barone G, Tucker ER, Marshall L, Herold R, Sterba J, Norga K, Vassal G, Pearson AD. Accelerating drug development for neuroblastoma - New drug development strategy: An innovative therapies for children with cancer, European network for cancer research in children and adolescents and International Society of Paediatric Oncology Europe neuroblastoma project. Expert Opin Drug Discov. 2017; 12: 801-11.

Nolan JC, Frawley T, Tighe J, Soh H, Curtin C, Piskareva O. Preclinical models for neuroblastoma: Advances and challenges. Cancer Lett. 2020; 474: 53-62.

Quinn PJ. Mechanisms of action of some immunomodulators used in veterinary medicine. Adv Vet Sci Comp Med. 1990; 35: 43-99.

Sachdev E, Tabatabai R, Roy V, Rimel BJ, Mita MM. PARP Inhibition in cancer: An update on clinical development. Target Oncol. 2019; 14: 657-79.

Sima P, Richter J, Vetvicka V. Glucans as new anticancer agents. Anticancer Res. 2019; 39: 3373-78.

Suebsoonthron J, Jaroonwitchawan T, Yamabhai M, Noisa P. Inhibition of WNT signaling reduces differentiation and induces sensitivity to doxorubicin in human malignant neuroblastoma SH-SY5Y cells. Anticancer Drugs. 2017; 28: 469-79.

Tang H, Zhang Y, Li D, Fu S, Tang M, Wan L, Chen K, Liu Z, Xue L, Peng A, Ye H, Chen L. Discovery and synthesis of novel magnolol derivatives with potent anticancer activity in non-small cell lung cancer. Eur J Med Chem. 2018; 156: 190-205.

Taskiran A S, Ergul M. Oxytocin promotes C6 glial cell death and aggravates hydrogen peroxide-induced oxidative stress. INNOSC Theranostics Pharmacol Sci. 2020 ; 3: 27-33.

Taskiran AS, Ergul M. The modulator action of thiamine against pentylenetetrazole-induced seizures, apoptosis, nitric oxide, and oxidative stress in rats and SH-SY5Y neuronal cell line. Chem Biol Interact. 2021; 340: 109447.

Vannucci L, Krizan J, Sima P, Stakheev D, Caja F, Rajsiglova L, Horak V, Saieh M. Immunostimulatory properties and anti-tumor activities of glucans (review). Int J Oncol. 2013; 43: 357-64.

Xu HL, Dai JH, Hu T, Liao YF. Lentinan up-regulates micro-RNA-340 to promote apoptosis and autophagy of human osteosarcoma cells. Int J Clin Exp Pathol. 2018; 11: 3876-83.

Xu H, Zou S, Xu X, Zhang L. Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism. Sci Rep. 2016; 6: 28802.

Zhang L, Li X, Xu X, Zeng F. Correlation between antitumor activity, molecular weight, and conformation of lentinan. Carbohydr Res. 2005; 340: 1515-21.

Zhang M, Chiu LC, Cheung PC, Ooi VE. Growth-inhibitory effects of a beta-glucan from the mycelium of Poria cocos on human breast carcinoma MCF-7 cells: Cell-cycle arrest and apoptosis induction. Oncol Rep. 2006; 15: 637-43.

Zhang X, Li T, Liu S, Xu Y, Meng M, Li X, Lin Z, Wu Q, Xue Y, Pan Y, Alitongbieke G. β-glucan from Lentinus edodes inhibits breast cancer progression via the Nur77/HIF-1α axis. Biosci Rep. 2020; 40: BSR20201006.

Zhang Y, Li Q, Wang J, Cheng F, Huang X, Cheng Y, Wang K. Polysaccharide from Lentinus edodes combined with oxaliplatin possesses the synergy and attenuation effect in hepatocellular carcinoma. Cancer Lett. 2016; 377: 117-25.


Apply citation style format of Bangladesh Journal of Pharmacology

Research Articles
Financial Support
Conflict of Interest
Authors declare no conflict of interest
Video Clip of Methodology