Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

  • Fahmida Hasnat Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.
  • Habiba Akhter Bhuiyan Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.
  • Mir Misbahuddin Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, Bangladesh. http://orcid.org/0000-0003-4667-1005
Keywords: High Performance Liquid Chromatography, Plasma, Vitamin B12
DOI: 10.3329/bjp.v12i3.33108

References

Gentili A, Caretti F, D'Ascenzo G, Marchese S, Perret D, Di Corcia D, Rocca LM. Simultaneous determination of waterâ€soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2008; 22: 2029-43.

Girdwood RH. Rapid estimation of the serum vitamin B12 level by a microbiological method. BMJ. 1954; 2(4894): 954-56.

Giorgi MG, Howland K, Martin C, Bonner B. A novel HPLC method for the concurrent analysis and quantitation of seven water-soluble vitamins in biological fluids (plasma and urine): A validation study and application. Sci World J. 2012.

Hancewicz TM, Petty C. Quantitative analysis of vitamin a using Fourier transform Raman spectroscopy. Spectrochim Acta A Mol Spectrosc. 1995; 51: 2193–98.

Heudi O, Kilinc T, Fontannaz P. Separation of water-soluble vitamins by reversed-phase high performance liquid chromatography with ultraviolet detection: Application to polyvitaminated premixes. J Chromatogr A. 2005; 1070: 49-56.

Karmi O, Zayed A, Baraghethi S, Qadi M, Ghanem R. Measurement of vitamin B12 concentration: A review on available methods. IIOAB J. 2011; 2: 23-32.

Klejdus B, Petrlová J, Potěšil D, Adam V, Mikelová R, Vacek J, Kizek R, Kubáň V., Simultaneous determination of water-and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Anal Chim Acta. 2004; 520: 57-67.

Liu BS, Gao J, Yang GL. Determination of vitamin B12 concentration by fluorescence quenching with acridine orange-rhodamine 6G energy transfer system. Guang Pu Xue Yu Guang Pu Fen Xi. 2005; 25: 1080–82.

Mayer E, Gardiner DJ, Hester RE. Resonance Raman spectra of vitamin B12 and dicyanocobala-min. Biochim Biophys Acta Gen Sub. 1973; 297: 568-70.

Sami R, Li Y, Qi B, Wang S, Zhang Q, Han F, Ma Y, Jing J, Jiang L. HPLC analysis of water-soluble vitamins (B2, B3, B6, B12, and C) and fat-soluble vitamins (E, K, D, A, and β-carotene) of okra (Abelmoschus esculentus). J Chem. 2014.

Shabangi M, Sutton JA. Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins. J Pharm Biomed Anal. 2005; 38: 66-71.

Shaik MM, Gan SH. Rapid resolution liquid chromatography method development and validation for simultaneous determination of homocysteine, vitamins B6, B9, and B12 in human serum. Indian J Pharmacol. 2013; 45: 159-67.

Song Z, Hou S. Subpicogram determination of vitamin B12 in pharmaceuticals and human serum using flow injection with chemiluminescence detection. Anal Chim Acta. 2003; 488: 71–79.

Stefova M, Stafilov T, Stojanosk, K, Cepreganova-Krstic B. Determination of vitamin B12 in multivitamin tablets by high performance liquid chromatography. Anal Lett. 1997; 30: 2723-31.

Yin C, Cao Y, Ding S, Wang Y. 2008. Rapid determination of water-and fat-soluble vitamins with microemulsion electrokinetic chromatography. J Chromatogr A. 2008; 1193: 172-77.

Published
2017-07-12

Apply citation style format of Bangladesh Journal of Pharmacology

Section
Visual Experiment
Financial Support
Self-funded
Conflict of Interest
Authors declare no conflict of interest
Video Clip of Methodology